Journal: PLoS ONE

Article Title: Circular RNA expression profiling of human granulosa cells during maternal aging reveals novel transcripts associated with assisted reproductive technology outcomes

doi: 10.1371/journal.pone.0177888

Figure Lengend Snippet: CircRNA_103827 and circRNA_104816 expressions in granulosa cells according to patients’ clinical characteristics.

Article Snippet: In stage 1, GCs samples from women of advanced age (AA, ≥ 38 years, n = 3) and young age (YA, ≤ 30 years, n = 3) were analyzed by a human circRNA microarray (8×15K, Arraystar Inc.).

Techniques: Biomarker Discovery

Journal: PLoS ONE

Article Title: Circular RNA expression profiling of human granulosa cells during maternal aging reveals novel transcripts associated with assisted reproductive technology outcomes

doi: 10.1371/journal.pone.0177888

Figure Lengend Snippet: CircRNA_103827 and circRNA_104816 expression levels in granulosa cells according to ART outcomes.

Article Snippet: In stage 1, GCs samples from women of advanced age (AA, ≥ 38 years, n = 3) and young age (YA, ≤ 30 years, n = 3) were analyzed by a human circRNA microarray (8×15K, Arraystar Inc.).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Circular RNA expression profiling of human granulosa cells during maternal aging reveals novel transcripts associated with assisted reproductive technology outcomes

doi: 10.1371/journal.pone.0177888

Figure Lengend Snippet: The top 10 significantly differentially expressed circRNAs between young and older women ranked by fold change.

Article Snippet: In stage 1, GCs samples from women of advanced age (AA, ≥ 38 years, n = 3) and young age (YA, ≤ 30 years, n = 3) were analyzed by a human circRNA microarray (8×15K, Arraystar Inc.).

Techniques:

Journal: PLoS ONE

Article Title: Circular RNA expression profiling of human granulosa cells during maternal aging reveals novel transcripts associated with assisted reproductive technology outcomes

doi: 10.1371/journal.pone.0177888

Figure Lengend Snippet: Comparison of candidate circRNAs (circRNA_103829, circRNA_10827, circRNA_104816, circRNA_101889, circRNA_103828, circRNA_100833, circRNA_104852, circRNA_103611) expression levels in granulosa cells from additional young (n = 20) and older (n = 20) women by qRT-PCR. YA, women with young age (≤ 30 years); AA, women with advanced age (≥ 38 years). P values were calculated by Mann-Whitney U test. Relative expressions were analyzed by 2 -ΔΔCt method which was normalized to GAPDH. For each box plotting, the central mark represents the median, the edges of the box represent the 25 th and 75 th percentiles, and the whiskers are the most extreme data points not considered outliers.

Article Snippet: In stage 1, GCs samples from women of advanced age (AA, ≥ 38 years, n = 3) and young age (YA, ≤ 30 years, n = 3) were analyzed by a human circRNA microarray (8×15K, Arraystar Inc.).

Techniques: Comparison, Expressing, Quantitative RT-PCR, MANN-WHITNEY

Journal: PLoS ONE

Article Title: Circular RNA expression profiling of human granulosa cells during maternal aging reveals novel transcripts associated with assisted reproductive technology outcomes

doi: 10.1371/journal.pone.0177888

Figure Lengend Snippet: ( A-E ) circRNA_103827 expression levels in granulosa cells according to (A) serum AMH levels, (B) AFC, (C) retrieved oocytes, (D) top quality embryos and (E) top quality embryo percentage. ( F-J ) circRNA_104816 expression levels in granulosa cells according to (F) serum AMH levels, (G) AFC, (H) retrieved oocytes, (I) top quality embryos and (J) top quality embryo percentage.

Article Snippet: In stage 1, GCs samples from women of advanced age (AA, ≥ 38 years, n = 3) and young age (YA, ≤ 30 years, n = 3) were analyzed by a human circRNA microarray (8×15K, Arraystar Inc.).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Circular RNA expression profiling of human granulosa cells during maternal aging reveals novel transcripts associated with assisted reproductive technology outcomes

doi: 10.1371/journal.pone.0177888

Figure Lengend Snippet: ROC analysis to evaluate predictive performance of circRNA_103827, circRNA_104816 expressions in granulosa cells and top quality embryo proportion for ( A-C ) clinical pregnancy outcomes, as well as for ( D-F ) live births. AUC, area under the ROC curve.

Article Snippet: In stage 1, GCs samples from women of advanced age (AA, ≥ 38 years, n = 3) and young age (YA, ≤ 30 years, n = 3) were analyzed by a human circRNA microarray (8×15K, Arraystar Inc.).

Techniques:

Journal: PLoS ONE

Article Title: Circular RNA expression profiling of human granulosa cells during maternal aging reveals novel transcripts associated with assisted reproductive technology outcomes

doi: 10.1371/journal.pone.0177888

Figure Lengend Snippet: (A) CircRNA_103827/circRNA_104816 targeted “Top 5” miRNA-gene network was portrayed based on sequence-pairing prediction. A pink round node represents a gene, a blue square represents miRNA, and a yellow diamond represents a circRNA. Overlapping genes of both circRNAs in this interactive network were ANKRD20A9P , XIST and KCNQ1OT1 . (B) GO and (C) KEGG pathway analysis of circRNA_103827 and circRNA_104816 predicted target genes. The top 10 significantly enriched activities and their scores (negative logarithm of P value) were listed in the X-axis and the Y-axis, respectively.

Article Snippet: In stage 1, GCs samples from women of advanced age (AA, ≥ 38 years, n = 3) and young age (YA, ≤ 30 years, n = 3) were analyzed by a human circRNA microarray (8×15K, Arraystar Inc.).

Techniques: Sequencing

Journal: Cancer Management and Research

Article Title: The Maturation of Tumor Suppressor miR-497 in Hepatocellular Carcinoma is Inhibited by Oncogenic circRNA SCARB1

doi: 10.2147/CMAR.S304125

Figure Lengend Snippet: The expression of circRNA SCARB1, mature miR-497 and miR-497 precursor was altered in HCC. HCC and paired non-tumor tissues were collected from the 64 HCC patients included in this study, and the expression of circRNA SCARB1 ( A ), mature miR-497 ( B ) and miR-497 precursor ( C ) in these tissue samples was determined by RT-qPCR. Average values of three technical replicates were used to express gene expression data in paired HCC and non-tumor tissues, ** p < 0.01.

Article Snippet: To overexpress circRNA SCARB1, pcDNA3.1(+) CircRNA Mini Vector (Addgene) was used as the backbone to construct the expression vector of circRNA SCARB1.

Techniques: Expressing, Quantitative RT-PCR, Gene Expression

Journal: Cancer Management and Research

Article Title: The Maturation of Tumor Suppressor miR-497 in Hepatocellular Carcinoma is Inhibited by Oncogenic circRNA SCARB1

doi: 10.2147/CMAR.S304125

Figure Lengend Snippet: The Correlations Between Clinicopathological Variables and the Expression of circRNA SCARB1 in HCC

Article Snippet: To overexpress circRNA SCARB1, pcDNA3.1(+) CircRNA Mini Vector (Addgene) was used as the backbone to construct the expression vector of circRNA SCARB1.

Techniques: Expressing, Infection

Journal: Cancer Management and Research

Article Title: The Maturation of Tumor Suppressor miR-497 in Hepatocellular Carcinoma is Inhibited by Oncogenic circRNA SCARB1

doi: 10.2147/CMAR.S304125

Figure Lengend Snippet: CircRNA SCARB1 and mature miR-497 were inversely correlated across HCC samples. Pearson’s correlation coefficient analysis was used to analyze the correlations between circRNA SCARB1 and mature miR-497 ( A ) or miR-497 precursor ( B ) across HCC tissue samples.

Article Snippet: To overexpress circRNA SCARB1, pcDNA3.1(+) CircRNA Mini Vector (Addgene) was used as the backbone to construct the expression vector of circRNA SCARB1.

Techniques:

Journal: Cancer Management and Research

Article Title: The Maturation of Tumor Suppressor miR-497 in Hepatocellular Carcinoma is Inhibited by Oncogenic circRNA SCARB1

doi: 10.2147/CMAR.S304125

Figure Lengend Snippet: Overexpression of circRNA SCARB1 downregulated mature miR-497 in HCC cells. To explore the effects of overexpression of circRNA SCARB1 on the maturation of miR-497, SNU-423 and SNU-387 cells were transfected with either circRNA SCARB1 expression vector or miR-497 mimic, followed by the confirmation of transfections at 48 h post-transfection by RT-qPCR ( A ). The effects of overexpression of circRNA SCARB1 of the expression of mature miR-497 ( B ) and miR-497 precursor ( C ), as well as the effects of the transfection of miR-497 mimic on circRNA SCARB1 expression ( D ) were also analyzed by RT-qPCR. Mean ± SD values were used to express data of 3 biological replicates of in vitro cell experiments. * p < 0.05.

Article Snippet: To overexpress circRNA SCARB1, pcDNA3.1(+) CircRNA Mini Vector (Addgene) was used as the backbone to construct the expression vector of circRNA SCARB1.

Techniques: Over Expression, Transfection, Expressing, Plasmid Preparation, Quantitative RT-PCR, In Vitro

Journal: Cancer Management and Research

Article Title: The Maturation of Tumor Suppressor miR-497 in Hepatocellular Carcinoma is Inhibited by Oncogenic circRNA SCARB1

doi: 10.2147/CMAR.S304125

Figure Lengend Snippet: Overexpression of circRNA SCARB1 increased HCC cell proliferation through miR-497. CCK-8 assay was performed to analyze the effects of overexpression of circRNA SCARB1 and miR-497 on the proliferation of SNU-423 ( A ) and SNU-387 cells ( B ). Mean ± SD values were used to express data of three biological replicates of in vitro cell experiments. * p < 0.05.

Article Snippet: To overexpress circRNA SCARB1, pcDNA3.1(+) CircRNA Mini Vector (Addgene) was used as the backbone to construct the expression vector of circRNA SCARB1.

Techniques: Over Expression, CCK-8 Assay, In Vitro

Journal: Cancer Management and Research

Article Title: The Maturation of Tumor Suppressor miR-497 in Hepatocellular Carcinoma is Inhibited by Oncogenic circRNA SCARB1

doi: 10.2147/CMAR.S304125

Figure Lengend Snippet: Overexpression of circRNA SCARB1 increased HCC cell migration through miR-497. Transwell assay was conducted to analyze the effects of overexpression of circRNA SCARB1 and miR-497 on the migration of SNU-423 ( A and C ) and SNU-387 cells ( B and D ). Mean ± SD values were used to express data of three biological replicates of in vitro cell experiments. * p < 0.05.

Article Snippet: To overexpress circRNA SCARB1, pcDNA3.1(+) CircRNA Mini Vector (Addgene) was used as the backbone to construct the expression vector of circRNA SCARB1.

Techniques: Over Expression, Migration, Transwell Assay, In Vitro

Journal: Journal of Cellular and Molecular Medicine

Article Title: Circular RNA circANAPC2 mediates the impairment of endochondral ossification by miR‐874‐3p/SMAD3 signalling pathway in idiopathic short stature

doi: 10.1111/jcmm.16419

Figure Lengend Snippet: Microarray analysis of differentially expressed circRNAs. A, Four pairs of ISS patients and age‐/sex‐matched controls were screened via circRNA microarray analysis. Thirty differentially expressed circRNAs were shown, including top 22 up‐regulated circRNAs and top 8 down‐regulated circRNAs. B, 145 circRNAs were significantly differentially expressed, including 83 up‐regulation and 62 down‐regulation. C, D, The top 30 KEGG pathway and GO enrichment are exhibited. The ubiquitin‐mediated proteolysis and Wnt signalling pathway are closely associated with chondrocyte proliferation, hypertrophy and endochondral ossification. CircRNAs, circular RNAs; KEGG, Kyoto Encyclopedia of Genes and Genomes; GO, Gene ontology; and Wnt, wingless/integrated

Article Snippet: The labelled coding RNAs were then hybridized onto the Human circRNAs Array v2 (8 × 15 K, Arraystar).

Techniques: Microarray

Journal: Molecular Therapy. Nucleic Acids

Article Title: hsa_circNFXL1_009 modulates apoptosis, proliferation, migration, and potassium channel activation in pulmonary hypertension

doi: 10.1016/j.omtn.2020.09.029

Figure Lengend Snippet: circRNA profile, the genome-wide landscape view, and hierarchical clustering of PAH-COPD patients and healthy controls (A) Volcano plot shows the different expression of circRNAs in two groups of subjects. Red/green dots indicate circRNAs expressed more/less than a 1.5-fold change in PAH-COPD patients. (B) Numbers of dysregulated circRNAs coming from different chromosomes. (C) Pie chart represents the percentage of dysregulated circRNAs coming from different chromosome regions. (D) Heatmap of 158 most significantly dysregulated circRNAs. (E) Heatmap of the top 40 dysregulated circRNAs (20 upregulated and 20 downregulated). Green/red represent a low/high expression level. PAH-COPD, pulmonary arterial hypertension due to chronic obstructive pulmonary disease; circRNA, circular RNA. n = 3 pairs of subjects. Comparisons of data were acquired by a Student’s t test.

Article Snippet: The circRNAs were transcribed into fluorescent cRNA and hybridized onto the Arraystar human circRNA array (8 ×15K, Arraystar) using a random priming method.

Techniques: Genome Wide, Expressing

Journal: Cancer Management and Research

Article Title: Profiles of differentially expressed circRNAs in esophageal and breast cancer

doi: 10.2147/CMAR.S167863

Figure Lengend Snippet: General information of ESCC patients for microarray test

Article Snippet: The RNA was isolated with an RNeasy mini kit (Qiagen, Hilden, Germany) and analyzed using an 8*15K Arraystar human circRNA microarray V2 (Catalog No: AS-CR-H-V2.0).

Techniques: Microarray

Journal: Cancer Management and Research

Article Title: Profiles of differentially expressed circRNAs in esophageal and breast cancer

doi: 10.2147/CMAR.S167863

Figure Lengend Snippet: General information of BC patients for microarray test

Article Snippet: The RNA was isolated with an RNeasy mini kit (Qiagen, Hilden, Germany) and analyzed using an 8*15K Arraystar human circRNA microarray V2 (Catalog No: AS-CR-H-V2.0).

Techniques: Microarray